Answering Common Questions Regarding Culture Media in PETG Media Bottles

Cell growth relies on culture media and other nutrients, typically sourced from serum. As a manufacturer of PETG Media Bottles, Fuda Cell has received numerous inquiries from customers regarding issues encountered during cell culture. Here are some insights to share:

Cell culture plays a crucial role in various biomanufacturing fields such as pharmaceuticals and vaccine development. Cell growth relies on culture media and other nutrients, typically sourced from serum. As a manufacturer of PETG Media Bottles, Fuda Cell has received numerous inquiries from customers regarding issues encountered during cell culture. Here are some insights to share:

How do we select the appropriate culture media corresponding to the serum stored in PETG Media Bottles?
Answer: There are no fixed culture conditions for cultivating a specific type of cell. Cells cultured in MEM may also thrive in DMEM or M199. In general, MEM is preferred for adherent cell culture, while RPMI-1640 is a good choice for suspension cell culture.

When should we add the culture media from PETG Media Bottles during cell culture?
Answer: It depends on the cell growth density or follows the recommended change time based on basic data of the cell line. Add the culture media from PETG Media Bottles to the cell culture consumables on time.

Is it permissible to replace the culture media in PETG Media Bottles midway through cell culture?
Answer: No. Each cell line has its specific and adapted culture media. If suddenly exposed to culture media different from the previously provided conditions, most cells cannot adapt immediately, resulting in cell death.

Can we change the type of serum in PETG Media Bottles?
Answer: No. Serum is a vital nutrient source for cell culture, and the type and quality of serum have a significant impact on cell growth. Serum from different species may differ in certain substances or molecules, and incorrect serum usage often leads to cell death.

What do the markings FBS, FCS, CS, HS on PETG Media Bottles from the manufacturer mean?
Answer: FBS (fetal bovine serum) and FCS (fetal calf serum) are the same, both referring to fetal bovine serum. The term FCS is incorrect and should be avoided. CS (calf serum) refers to calf serum. HS (horse serum) refers to horse serum.

These are some common issues encountered during cell culture regarding the culture media and serum stored in PETG Media Bottles. We hope this information is helpful.