Cells That Can Be Cultured Using the Cell Factory Analogy

The cell factory analogy, as an advanced cell culture platform, is designed with a multi-layer structure, making it ideal for large-scale production and culture of various cell types.

The cell factory analogy, as an advanced cell culture platform, is designed with a multi-layer structure, making it ideal for large-scale production and culture of various cell types. Its wide range of applications spans biopharmaceuticals, vaccine production, and cell therapy. Below are some common cell types that can be cultured using this system:

1. Mammalian Cells
CHO Cells (Chinese Hamster Ovary Cells): Widely used in the production of biopharmaceuticals, especially in recombinant protein and monoclonal antibody production.
HEK293 Cells (Human Embryonic Kidney Cells): Commonly used in gene expression research, vaccine production, and recombinant protein production.
BHK Cells (Baby Hamster Kidney Cells): Used in vaccine production, virology research, and protein production.
NS0 Cells (Mouse Myeloma Cells): Employed in monoclonal antibody production.
2. Insect Cells
Sf9 Cells and High Five Cells: Frequently used for expressing foreign proteins, particularly in insect cell expression systems like the Baculovirus (BAC) system.
3. Plant Cells
Tobacco Cells (e.g., BY-2 Cell Line): Used for the production of plant-based pharmaceuticals and the synthesis of secondary metabolites.

4. Cells for Cell Therapy
Stem Cells: Including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), utilized in regenerative medicine and cell therapies.
T Cells: Such as CAR-T cells, applied in cancer immunotherapy.
Mesenchymal Stem Cells (MSCs): Used for the treatment of various diseases, including joint disorders and cardiovascular diseases.
5. Cells for Vaccine Production
Vero Cells: Used to produce a variety of vaccines, including influenza and polio vaccines.
MDCK Cells (Madin-Darby Canine Kidney Cells): Employed in the production of influenza vaccines.
6. Cells for Virus Production
293T Cells: Commonly used for the production of viral vectors, such as adenovirus and lentivirus.
PER.C6 Cells: Utilized in the production of vaccines and therapeutic proteins.
7. Other Cell Lines
RTG-2 Cells (Rainbow Trout Gonad Cells): Used in the study of fish viruses and other aquatic pathogens.
Conclusion
The multi-layer design of the cell factory analogy provides an extensive surface area for cell culture, enabling high-density growth within a compact container volume. This design enhances cell growth and product yield, making it efficient for various applications. While commonly used for the aforementioned cell types, the platform is also suitable for cultivating and producing other specialized cell lines, contributing significantly to advancements in life sciences.